Complementation studies can be performed in bacteria by
A)gene targeting.
B)forming merodiploids with one copy of a bacterial gene on an F′ plasmid.
C)forming merodiploids with two copies of a bacterial gene on F′ plasmids.
D)forming diploids with two copies of the bacterial chromosome.

Question

Quizplus · Accepted Answer

Complementation studies in bacteria often involve forming merodiploids, which are partially diploid for certain genes. This is achieved by introducing an F′ plasmid carrying a copy of the gene of interest into a bacterium. If the gene on the plasmid complements a mutation in the bacterial chromosome, it can restore the function lost due to the mutation, allowing for the study of gene function and interaction.

Complementation Studies Can Be Performed in Bacteria by