To generate a knockin mouse, a scientist introduces to ES cells a construct with a mutant exon and within an adjacent intron, loxP sites flanking a neomycin resistance gene.What event will occur next?
A)Homologous recombination between the mutant DNA and the corresponding gene in the mouse genome.
B)Recombination between the loxP sites.
C)Transplantation of cells into host blastocyst.
D)Crossing of heterozygous mice to a Cre-expressing strain.

Question

Quizplus · Accepted Answer

Homologous recombination between the mutant DNA and the corresponding gene in the mouse genome is the next step after introducing the construct into ES cells. This process allows the mutant exon to replace the corresponding sequence in the mouse genome, ensuring the knockin mutation is incorporated.

To Generate a Knockin Mouse, a Scientist Introduces to ES