Which of the following would you expect to observe in an in vitro experimental mixture containing microtubules anchored by their minus ends to centrosomes,polystyrene beads with kinesin or dynein motors attached,and ATP?
A)movement of kinesin-attached beads along microtubules away from centrosomes
B)movement of dynein-attached beads along microtubules toward from centrosomes
C)movement of beads in either direction along a single microtubule
D)removal of ATP stopping all bead movement along microtubules
E)All would be expected.

Question

Quizplus · Accepted Answer

Kinesin typically moves towards the plus end of microtubules, which would be away from the centrosomes when microtubules are anchored by their minus ends. Dynein moves towards the minus end, which would be towards the centrosomes under these conditions. Movement in either direction could occur depending on which motor protein is attached to a bead. Removal of ATP would stop all movement since ATP is required for the motor proteins' activity.

Which of the Following Would You Expect to Observe in an in Vitro