What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
A)Transform bacteria with a recombinant DNA molecule.Cut the plasmid DNA using restriction enzymes.Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.Extract plasmid DNA from bacterial cells.Use ligase to seal plasmid DNA to nonplasmid DNA.
B)Cut the plasmid DNA using restriction enzymes.Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.Use ligase to seal plasmid DNA to nonplasmid DNA.Extract plasmid DNA from bacterial cells.Transform bacteria with a recombinant DNA molecule.
C)Extract plasmid DNA from bacterial cells.Cut the plasmid DNA using restriction enzymes.Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.Use ligase to seal plasmid DNA to nonplasmid DNA.Transform bacteria with a recombinant DNA molecule.
D)Extract plasmid DNA from bacterial cells.Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.Use ligase to seal plasmid DNA to nonplasmid DNA.Transform bacteria with a recombinant DNA molecule.Cut the plasmid DNA using restriction enzymes.

Question

Quizplus · Accepted Answer

The correct sequence starts with extracting plasmid DNA from bacterial cells, then cutting the plasmid DNA with restriction enzymes to create sticky ends. Next, the plasmid DNA is hydrogen-bonded to nonplasmid DNA fragments (foreign DNA) that have complementary sticky ends. The DNA fragments are then permanently joined using DNA ligase. Finally, the recombinant DNA molecule is introduced into bacteria through a process called transformation.

What Is the Most Logical Sequence of Steps for Splicing