Which of the following procedures would identify the enzyme that catalyzes the carboxylation of ribulose- 1,5- bisphosphate?
A) differential sedimentation of a protein extract
B) introducing radiolabeled carbon dioxide into a plant extract and determining which molecules become radiolabeled
C) irradiating a leaf extract with red light
D) purifying a variety of proteins from plant extracts and testing each protein individually to see if it can carboxylate ribulose- 1,5- bisphosphate

Question

Quizplus · Accepted Answer

Purifying and testing proteins individually allows for the identification of the specific enzyme responsible for the carboxylation of ribulose-1,5-bisphosphate, which is the function of the enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO).

Which of the Following Procedures Would Identify the Enzyme That