CD34 enumeration is useful in bone marrow and peripheral blood stem cell transplantation. For this enumeration, the EWGCCA recommendations include proper technique and interpretation of the flow cytometry analysis. Which of the following standards was recommended for proper acquisition of events?
A) Include platelets and unlysed red blood cells and debris
B) Include only CD34 dim staining populations
C) Use bright fluorochrome conjugates of class II or III monoclonal antibodies that detect all glycoforms of CD34
D) Acquire only 10 CD34 positive cells

Question

Quizplus · Accepted Answer

The EWGCCA recommends the use of bright fluorochrome conjugates of class II or III monoclonal antibodies that detect all glycoforms of CD34. This ensures better sensitivity and specificity in detecting CD34 positive cells. Including platelets and unlysed red blood cells and debris (A) can lead to background noise and inaccurate results. Acquiring only CD34 dim staining populations (B) can lead to the exclusion of CD34 bright staining populations that may be important for enumeration. Acquiring only 10 CD34 positive cells (D) is not sufficient for accurate enumeration.

CD34 Enumeration Is Useful in Bone Marrow and Peripheral Blood