Deck 63: Overview of the Methods and Strategies in Virology

Question 1

Which assay is the current recommended method of detection in most laboratories for influenza A and B viruses?

A)Conventional cell culture
B)Enzyme immunoassays
C)Reverse transcriptase polymerase chain reaction (RT-PCR)
D)Shell vial culture

Answer 1

C
Influenza A and B viruses can be detected by using conventional cell culture,shell vial culture,membrane enzyme immunoassay (EIA),direct staining of respiratory tract secretions using fluorescent-antibody (FA)methods ,and RT-PCR.RT-PCR is the current recommended method of detection in most laboratories.

Question 2

A 2-year-old girl is suspected of having a rotavirus infection.The specimen of choice to collect to identify the agent is:

A)stool.
B)blood.
C)urine.
D)throat.

Answer 2

A
Stool and rectal swabs of fecal specimens are used to detect rotavirus,enteric adenoviruses (serotypes 40 and 41),and enteroviruses.

Question 3

The most used EIA for antigen detection that uses a solid phase and membrane is used for detecting which virus?

A)Influenza
B)Adenovirus
C)HSV
D)Respiratory syncytial virus (RSV)

Answer 3

D
The most used EIA for antigen detection are those for RSV (solid phase and membrane),rotavirus (solid phase and membrane),and influenza viruses (membrane).

Question 4

To detect immunoglobulin M (IgM)antibodies in an acute phase,blood should be collected in _____ days after the onset of infection.

A)2 to 5
B)5 to 7
C)7 to 14
D)longer than 21

Question 5

If a virus is referred to as naked,it means it lacks which structure?

A)Protein coat
B)Capsid
C)Envelope
D)Nucleocapsid

Question 6

MATCHING
Match the virus with the specimen in which it is most commonly isolated.
a.Varicella-zoster virus
b.Enterovirus
c.Human papillomavirus (HPV)
d.Parainfluenza virus
e.CMV
Nasopharyngeal

Question 7

MATCHING
Match the virus with the specimen in which it is most commonly isolated.
a.Varicella-zoster virus
b.Enterovirus
c.Human papillomavirus (HPV)
d.Parainfluenza virus
e.CMV
Stool

Question 8

MATCHING
Match the virus with the specimen in which it is most commonly isolated.
a.Varicella-zoster virus
b.Enterovirus
c.Human papillomavirus (HPV)
d.Parainfluenza virus
e.CMV
Genital

Question 9

If Negri bodies are identified in brain tissue through cytologic means,then the virus is identified as a(n):

A)adenovirus.
B)parvovirus.
C)rabies virus.
D)varicella virus.

Question 10

Some viruses have the ability to stimulate uncontrolled growth of the host cells,resulting in cancer.Which virus has oncogenic properties?

A)Measles
B)Papilloma
C)Retroviruses
D)Influenza

Question 11

The function of the glycoprotein spike on some viruses is to:

A)allow viral entry into the host cell.
B)protect the nucleic acid.
C)prevent drying out and the destruction of the virus.
D)stabilize attachment for the lipid envelope.

Question 12

MATCHING
Match the virus with the specimen in which it is most commonly isolated.
a.Varicella-zoster virus
b.Enterovirus
c.Human papillomavirus (HPV)
d.Parainfluenza virus
e.CMV
Skin and mucous membranes

Question 13

Which type of swab is not suitable for the collection of a viral specimen as a result of its ability to interfere with certain tests?

A)Dacron
B)Calcium alginate
C)Cotton
D)Rayon

Question 14

The enzyme-linked virus-inducible system (ELVIS)uses a cloned β-galactosidase gene that is expressed only when cells are infected with what virus?

A)RSV
B)Human immunodeficiency virus (HIV)
C)HSV
D)Cytomegalovirus (CMA)

Question 15

Amie's,Leibovitz-Emory,and Stuart's are all types of:

A)cell lines.
B)cell culture media.
C)automated systems.
D)transport media.

Question 16

MATCHING
Match the virus with the specimen in which it is most commonly isolated.
a.Varicella-zoster virus
b.Enterovirus
c.Human papillomavirus (HPV)
d.Parainfluenza virus
e.CMV
Urine

Question 17

The cytomegalovirus (CMV)produces cytopathic effects (CPE)in diploid fibroblast cells in an average of 7 days.The results are available within 16 hours using which type of assay?

A)Conventional cell culture
B)Shell vial
C)Paired sera
D)Western blot

Question 18

Match the virus with its corresponding property.
a.RSV
b.HIV
c.CMV
d.Adenovirus
e.HSV
f.Pediatric respiratory virus
g.Hepatitis B virus (HBV)
h.Influenzas A and B
pp65 protein

Question 19

The specimen of choice to collect in suspected cases of respiratory syncytial virus (RSV)is:

A)buccal mucosa.
B)sputum.
C)throat.
D)nasopharyngeal.

Question 20

The appropriate temperature in which to store specimens for viral isolation is ____°C.

A)4
B)25
C)30
D)37

Question 21

Match the virus with its corresponding property.
a.RSV
b.HIV
c.CMV
d.Adenovirus
e.HSV
f.Pediatric respiratory virus
g.Hepatitis B virus (HBV)
h.Influenzas A and B
MRC-5 cell lines

Question 22

Match the cells with the appropriate cell line.
a.HEp-2 culture cells
b.Monkey kidney cells
c.Fibroblast cells
Primary cell line

Question 23

Match the virus with its corresponding property.
a.RSV
b.HIV
c.CMV
d.Adenovirus
e.HSV
f.Pediatric respiratory virus
g.Hepatitis B virus (HBV)
h.Influenzas A and B
p24 protein

Question 24

Match the virus with the appropriate specimen.
a.Adenovirus
b.HSV
c.HBV
d.Arbovirus
Vesicular

Question 25

Match the virus with its corresponding property.
a.RSV
b.HIV
c.CMV
d.Adenovirus
e.HSV
f.Pediatric respiratory virus
g.Hepatitis B virus (HBV)
h.Influenzas A and B
Surface antigen

Question 26

Match the virus with its corresponding property.
a.RSV
b.HIV
c.CMV
d.Adenovirus
e.HSV
f.Pediatric respiratory virus
g.Hepatitis B virus (HBV)
h.Influenzas A and B
HEp-2 culture cells

Question 27

Match the virus with its corresponding property.
a.RSV
b.HIV
c.CMV
d.Adenovirus
e.HSV
f.Pediatric respiratory virus
g.Hepatitis B virus (HBV)
h.Influenzas A and B
Adenovirus serotypes 40 and 41

Question 28

Match the virus with its corresponding property.
a.RSV
b.HIV
c.CMV
d.Adenovirus
e.HSV
f.Pediatric respiratory virus
g.Hepatitis B virus (HBV)
h.Influenzas A and B
R-Mix cells

Question 29

Match the cells with the appropriate cell line.
a.HEp-2 culture cells
b.Monkey kidney cells
c.Fibroblast cells
Continuous cell line

Question 30

Match the cells with the appropriate cell line.
a.HEp-2 culture cells
b.Monkey kidney cells
c.Fibroblast cells
Diploid cell line

Question 31

Match the virus with the appropriate specimen.
a.Adenovirus
b.HSV
c.HBV
d.Arbovirus
Cerebrospinal fluid (CSF)antibody

Question 32

Match the virus with its corresponding property.
a.RSV
b.HIV
c.CMV
d.Adenovirus
e.HSV
f.Pediatric respiratory virus
g.Hepatitis B virus (HBV)
h.Influenzas A and B
Hemadsorption

Question 33

Match the virus with the appropriate specimen.
a.Adenovirus
b.HSV
c.HBV
d.Arbovirus
Respiratory

Question 34

Match the virus with the appropriate specimen.
a.Adenovirus
b.HSV
c.HBV
d.Arbovirus
Blood

Question 35

List these six steps of viral replication in order of appearance.
Assembly
Uncoating
Attachment
Release
Synthesis
Penetration