Deck 15: Component Preparation

Question 1

What dose of RhIG would be appropriate for a D-negative woman who has had a miscarriage at 11 weeks' gestation?

A) 300 µg
B) 120 µg
C) 50 µg
D) All of the above

Answer 1

C

Question 2

What is the shelf life of whole blood collected in citrate-phosphate-double dextrose?

A) 10 days
B) 21 days
C) 35 days
D) 50 days

Answer 2

B

Question 3

Which of the following statements explains why bacterial contamination of blood is rarely a problem?

A) Regulated procedures for arm preparation prevent most bacterial contamination from occurring during collection.
B) Citrate in the anticoagulant is not conducive to bacterial growth.
C) The cold storage of blood is not conducive to bacterial growth.
D) All of the above

Answer 3

D

Question 4

A patient has a baseline platelet count of 30,000/µL. Upon receiving a platelet pool of 4 random platelets, what would you expect the post-transfusion platelet count to be?

A) 35,000/µL
B) 50,000/µL
C) 80,000/µL
D) 100,000/µL

Question 5

Leukoreduced packed RBCs must have an absolute white blood cell count of less than and contain at least what percent of original RBC mass?

A) 5 $\times$ 10⁸/85
B) 5 $\times$ 10⁶/85
C) 5 $\times$ 10⁸/80
D) 5 $\times$ 10⁶/80

Question 6

Normally what percentage of 35-day-old red blood cells should be circulating 24 hours after transfusion?

A) 100%
B) 50%
C) 65%
D) 70%

Question 7

Methods of preparation of platelet concentrates from single units of whole blood must produce a product that yields a minimum of:

A) 5.5 $\times$ 10¹¹ platelets per unit in 50% of units tested.
B) 5.5 $\times$ 10¹¹ platelets per unit in 75% of units tested.
C) 5.5 $\times$ 10¹⁰ platelets per unit in 75% of units tested.
D) none of the above.

Question 8

What is the shelf life of whole blood collected in citrate-phosphate-dextrose-adenine?

A) 10 days
B) 21 days
C) 35 days
D) 50 days

Question 9

Packed RBCs must have a final hematocrit of less than or equal to:

A) 85%.
B) 38%.
C) 80%.
D) 70%.

Question 10

What is the shelf life of whole blood collected in acid-citrate-dextrose?

A) 10 days
B) 21 days
C) 35 days
D) 50 days

Question 11

All of the following statements are characteristic of a penetrating cryoprotective agent except:

A) it consists of generally small molecules.
B) osmotic force prevents migration of water outside of the cell, preventing dehydration.
C) large molecules form a shell around the cell, preventing loss of water and dehydration.
D) none of the above.

Question 12

What is the expiration time for platelet concentrates that have been pooled?

A) 24 hours
B) 6 hours
C) 4 hours
D) 48 hours

Question 13

A whole blood donation contains a volume of 350 mL. Which of the following is true regarding this unit?

A) Packed red blood cells cannot be made from this unit.
B) FFP cannot be made from this unit.
C) Cryoprecipitate can be made from this unit.
D) Platelets cannot be made from this unit.

Question 14

How are RBCs separated from whole blood?

A) Apheresis
B) Centrifugation
C) Sedimentation
D) All of these

Question 15

What is the minimal pH required for platelets?

A) 6.0
B) 6.2
C) 7.0
D) 7.2

Question 16

Within what time after collection of whole blood must RBCs be separated from whole blood in order for platelet and plasma components to be prepared?

A) Immediately
B) 2 to 5 hours
C) 8 to 24 hours
D) Any time

Question 17

A unit of whole blood must be stored at what temperature?

A) -65° to -20°C
B) - 10° to 2°C
C) 1° to 6°C
D) 8° to 20°C

Question 18

What is the ratio of anticoagulant to whole blood in a unit of whole blood?

A) 8 mL of anticoagulant-preservative for every 100 mL of whole blood collected
B) 14 mL of anticoagulant-preservative for every 100 mL of whole blood collected
C) 22 mL of anticoagulant-preservative for every 100 mL of whole blood collected
D) 30 mL of anticoagulant-preservative for every 100 mL of whole blood collected

Question 19

A unit of red blood cells has an expiration date of 11/15/12. A patient currently on a fludarabine regimen requires irradiated packed red blood cells. The unit is irradiated for this patient on 11/02/12. What is the correct expiration date postirradiation?

A) 11/15/12
B) 11/30/12
C) 11/28/12
D) 11/02/12

Question 20

Which of the following are approved preservative solutions for blood storage at 1°C to 6°C for 21 days?

A) ACD
B) CPD
C) CP2D
D) All of the above

Question 21

Manufacturing RDPs is accomplished by using all of the following methods EXCEPT:

A) apheresis collection.
B) buffy coat aspiration.
C) centrifuge on high
D) centrifuge on low.

Question 22

What is the shelf life of packed RBCs collected in acid-citrate-dextrose?

A) 10 days
B) 21 days
C) 35 days
D) 50 days

Question 23

Plasma factor concentrates are separated into various proteins by manipulating which variable?

A) pH
B) Alcohol content
C) Temperature
D) All of these

Question 24

What is the shelf-life of platelets if the unit is tested for bacterial contamination just prior to issuing?

A) 2 days
B) 5 days
C) 7 days
D) 10 days

Question 25

Frozen plasma is thawed at what temperature?

A) 2° to 4°C
B) 18° to 22°C
C) 30° to 37°C
D) 45° to 56°C

Question 26

A unit of granulocytes prepared on April 13^th at 10:00 am and is irradiated at 2:00 pm the same day. What is the expiration date?

A) April 13th 3:00 pm.
B) April 13th 10:00 pm.
C) April 14th 10:00 am.
D) April 14th 2:00 pm.

Question 27

Which of the following is/are an example of a penetrating cryoprotective agent?

A) Dimethylsulfoxide
B) Glycerol
C) Hydroxyethyl starch
D) All of these

Question 28

A unit of granulocyte should contain at least what concentration of granulocytes?

A) 1  1010
B) 4  1010
C) 6  1010
D) 10  1010

Question 29

Which statement about liquid plasma is correct?

A) Liquid plasma is a product from whole blood donations.
B) The expiration of liquid plasma is 5 days after its whole blood shelf-life.
C) Liquid plasma is stored at 1° to 6°C.
D) All of these are correct.

Question 30

Liquid plasma is prepared from a unit of whole blood collected in CPDA-1 on July 28^th. What is the expiration date of this plasma?

A) August 18th
B) August 23rd
C) September 2nd
D) September 7th

Question 31

A unit of cryopreserved RBCs must be stored below what temperature?

A) -80°C
B) -65°C
C) -20°C
D) 0°C

Question 32

What are platelets obtained from apheresis donations called?

A) Random-donor platelets (RDPs)
B) Single-donor platelets (SDPs)
C) Targeted-donor platelets (TDPs)
D) None of these

Question 33

Viruses are inactivated in units of plasma factor concentrate by all of these methods EXCEPT:

A) freezing.
B) heating.
C) solvent-detergent.
D) nanofiltration.

Question 34

What is the expiration date of a deglycerolized unit of RBCs?

A) 10 hours
B) 24 hours
C) 36 hours
D) 72 hours

Question 35

A unit of packed platelets must be stored at what temperature?

A) 1° to 6°C
B) 8° to 20°C
C) 20° to 24°C
D) 32° to 45°C

Question 36

Which statement about frozen plasma is correct?

A) The expiration of frozen plasma stored at -18°C is 10 years.
B) The expiration of frozen plasma stored at -65°C is 7 years.
C) The expiration of frozen plasma stored at -10° to 0°C is 5 years.
D) All of these are correct.

Question 37

Which factor concentrate has almost completely replaced cryoprecipitate as the product of choice to treat patients with hemophilia A?

A) FVIIa
B) rFVIIa
C) FVIII
D) FVIX

Question 38

A unit of packed RBCs must be stored at what temperature?

A) -65° to -20°C
B) - 10° to 2°C
C) 1° to 6°C
D) 8° to 20°C

Question 39

Units of platelets are stored under which of these conditions?

A) Constant agitation
B) Darkened chamber
C) Hanging from a hook
D) None of these

Question 40

What is the shelf-life of frozen RBCs?

A) 1 year
B) 5 years
C) 10 years
D) Indefinite

Question 41

What is the shelf-life for normal serum albumin?

A) 1 year
B) 3 years
C) 5 years
D) 10 years

Question 42

In which animal has transgenic methods produced rAT in the milk?

A) Cows
B) Goats
C) Pigs
D) Sheep

Question 43

Which of the follow is NOT considered in the preparation of Rho immunoglobulin?

A) Donors are Rh-(D) positive.
B) Donors have been hyperimmunized.
C) Donor plasmas contain IgG anti-D.
D) Donor human plasmas are pooled.

Question 44

What is the shelf-life for plasma protein fraction?

A) 3 years
B) 5 years
C) 10 years
D) 15 years

Question 45

What is the half-life of immune serum globulin?

A) 3 to 7 days
B) 10 to 15 days
C) 18 to 32 days
D) 40 to 45 days

Question 46

Which of the follow does NOT describe the preparation of immune serum globulin?

A) Immune serum globulin is a concentrate of plasma gamma globulins.
B) It is prepared from pooled plasma by cold ethanol fractionation.
C) Preparations include intravenous (IV) or intramuscular (IM) forms.
D) The solution of immune serum globulin is dehydrated state and must be reconstituted with saline.

Question 47

What is the storage temperature for plasma protein fraction?

A) -80° to -65°C
B) -20° to 0°C
C) 2° to 10°C
D) 20° to 25°C

Question 48

Xenographic forms of factor VIII are made from which source of plasma?

A) Bovine plasma
B) Human plasma
C) Porcine plasma
D) Reptilian plasma

Question 49

Which of the follow does NOT describe the preparation of antithrombin?

A) Apheresis of a single donor sensitized donor
B) Prepared from pooled human plasma
C) Viral inactivation using heat treatment
D) Purification of milk from transgenic goats.

Question 50

How is the preparation of PPF different than that of NSA?

A) PPF is salvaged and pooled; NSA is not pooled.
B) PPF is cold ethanol fractionated; NSA is warm alcohol fractionated.
C) PPF uses heat inactivation of viruses; NSA does not.
D) PPF contains less albumin and more globulins than NSA.

Question 51

What is the storage temperature for normal serum albumin?

A) -80° to -65°C
B) -20° to 0°C
C) 2° to 10°C
D) 20° to 25°C

Question 52

Which of the follow does NOT describe the preparation of NSA?

A) Plasma is salvaged and pooled.
B) Plasma is fractionated using a warm alcohol process.
C) HIV and hepatitis are inactivated with heat inactivation.
D) NSA composition includes of 96% albumin and 4% globulin.